An abscisic acid-responsive protein interaction network for sucrose non-fermenting related kinase1 in abiotic stress response

Carina Steliana Carianopol,Shaowei Dong,Shelley Lumba,Nicholas J Provart,Sonia Gazzarrini,Aaron Lorheed Chan

doi:10.1038/s42003-020-0866-8

Carina Steliana Carianopol, Shaowei Dong + Show 4 more

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https://doi.org/10.1038/s42003-020-0866-8

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Abstract

Yeast Snf1 (Sucrose non-fermenting1), mammalian AMPK (5′ AMP-activated protein kinase) and plant SnRK1 (Snf1-Related Kinase1) are conserved heterotrimeric kinase complexes that re-establish energy homeostasis following stress. The hormone abscisic acid (ABA) plays a crucial role in plant stress response. Activation of SnRK1 or ABA signaling results in overlapping transcriptional changes, suggesting these stress pathways share common targets. To investigate how SnRK1 and ABA interact during stress response in Arabidopsis thaliana, we screened the SnRK1 complex by yeast two-hybrid against a library of proteins encoded by 258 ABA-regulated genes. Here, we identify 125 SnRK1- interacting proteins (SnIPs). Network analysis indicates that a subset of SnIPs form signaling modules in response to abiotic stress. Functional studies show the involvement of SnRK1 and select SnIPs in abiotic stress responses. This targeted study uncovers the largest set of SnRK1 interactors, which can be used to further characterize SnRK1 role in plant survival under stress.

Highlights

Yeast Snf[1] (Sucrose non-fermenting1), mammalian AMPK (5′ AMP-activated protein kinase) and plant SnRK1 (Snf1-Related Kinase1) are conserved heterotrimeric kinase complexes that re-establish energy homeostasis following stress
To identify proteins that are regulated by abscisic acid (ABA) and SnRK1 and may play a role in plant stress responses, the six SnRK1 subunits (α1, α2, β1, β2, β3, and βγ) previously shown to form SnRK1 complexes in vivo[20,21,22] were screened against a library of proteins encoded by 258 ABA-regulated genes[5]
Environmental stress greatly affects plant productivity and poses a threat to food security. Abiotic stress such as osmotic, salt and drought leads to the accumulation of ABA, which promotes stress adaptation through the activation of stressspecific as well as shared signaling pathways[55]

Summary

Introduction

Yeast Snf[1] (Sucrose non-fermenting1), mammalian AMPK (5′ AMP-activated protein kinase) and plant SnRK1 (Snf1-Related Kinase1) are conserved heterotrimeric kinase complexes that re-establish energy homeostasis following stress. The hormone abscisic acid (ABA) plays a crucial role in plant stress response. Activation of SnRK1 or ABA signaling results in overlapping transcriptional changes, suggesting these stress pathways share common targets. Functional studies show the involvement of SnRK1 and select SnIPs in abiotic stress responses This targeted study uncovers the largest set of SnRK1 interactors, which can be used to further characterize SnRK1 role in plant survival under stress. SnRK1/Snf1/AMPK are conserved heterotrimeric kinase complexes that are activated under energy limitation to induce metabolic reprogramming and achieve energy homeostasis in plants, yeast, and mammals[7,8,9]. SnRK2/3 cannot functionally substitute for SnRK1, suggesting functional diversification[7]

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