Amylolytic-dependent biofilm inhibition of H. forrestti var. forrestii rhizome extract against S.aureus pathogens

Abstract

Rhizosphere bacterial infections are studied with great interest as it enlightens the idea of host-pathogen interaction combined with host defence adaptations. In this study, we are studying the ability of Hedychium forrestti var. forrestii rhizome against rhizosphere bacterial colonialization. It is understood that bacterial colonialization is immensely supported by biofilm production. We analyzed the biofilm inhibition efficacy of the plant rhizome extract, which suggested that the presence of plant rhizome extract could successfully downregulate the rate of bacterial adherence. The Spectrophotometrical data indicated that about 80% of biofilm production by Staphylococcus aureus can be inhibited by administering 500 µg/ml plant rhizome extract. The microscopic studies pointed out that antagonist-disabled bacteria form a film upon a given surface compared to untreated conditions. Fluorescence microscopy also identified that biofilm inhibition made bacteria more vulnerable. It was observed that the biofilm inhibition by plant rhizome extract is complimented by the amylolytic property of plant rhizome extract, verified by the enzyme kinetics studies. The plant rhizome extract process suggested a notable amylolytic action by Michaelis–Menten kinetics.