Abstract
A single-chain fragment variable (scFv) antibody library displayed on phage was constructed using spleen cells from mice immunized with human amyloid-beta peptide (Aβ42). This first anti-Aβ42 scFv immune antibody library was selected against human Aβ42. A number of positive clones were obtained, and sequences of V H and Vκ genes were analyzed using ExPASy and BLAST computer tools. This analysis revealed that only two unique clones with identical V H and Vκ complementarity determining region (CDR) (except HCDR2) and identical germline genes were selected, indicating that oligoclonal immune response was occurring in Aβ42-immunized mice. Aβ42-specific scFv antibodies selected from this first immune anti-Aβ42 phage antibody library may be an important tool for the development of therapeutic molecules for Alzheimer's disease (AD).
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