Amyloid Precursor Protein Influences Gallbladder Cancer Cell Behaviors and may be an Effective Prognostic Factor

Abstract

Gallbladder cancer is one of the most aggressive carcinomas of digestive system. The conventional surgical resection, radiotherapy and chemotherapy limit therapeutic effects on gallbladder cancer, so it is urgent to further explore the mechanism of the development of gallbladder cancer. To solve this problem, we have studied the application value of amyloid precursor protein (APP) in the diagnosis and treatment of gallbladder cancer. Small interfering ribonucleic acid (siRNA) was used to knock down APP in SGC-996 and QBC-939 cells. Then, the effects of APP on SGC-996 and QBC-939 cells proliferation, migration, invasion and cell cycle were evaluated. The results showed that APP was overexpressed in gallbladder cancer tissues and cell lines. The cell proliferation, migration and invasion were effectively inhibited and the cell cycle was influenced to a certain extent after knockdown of APP. And the expression of tumor metastatic associated protein, MMP-2, MMP-9, E-cadherin, N-cadherin, was associated with the level of APP. The present data confirmed that APP played a crucial role in gallbladder cancer cells function. This study strongly suggests that APP is a potential target for gallbladder cancer treatment.