Abstract

AbstractAmylase isozymes were studied in six varieties of Avena sativa L. using agar gel electrophoresis. Three distinct zymograms were detected in aqueous extracts of endosperm of 4 to 5‐day old seedlings. No intravarietal variation was observed. These results indicate that amylase zymograms may be useful markers for helping in the identification of oat varieties. Using the varieties Maris Tabard and Maris Titan it was found that all the isozymes in 4 to 5‐day old seedlings, except one, had the properties of α‐amylase. The zymograms of extracts from ungerminated grains of these two varieties, although apparently identical to those of 4 to 5‐day old seedlings, differed in that all the α‐isozymes in the former were found to be heat labile whereas in the latter they were heat stable.

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