Abstract
This study aimed at evaluating the ameliorative effect of amygdalin and magnetic water on 1,2-dimethyl hydrazine induced toxic damage in tissues and oxidative stress in rat liver. Seven groups of ten rats each were selected for the study. Group I animals were treated as control. Group II rats received 1,2-dimethyl hydrazine (20 mg/kg body weight) injections subcutaneously once a week for 16 consecutive weeks and then kept without any treatment untill the end of the experimental period. Group III rats received amygdalin (20 mg/100 mg) daily via Oro-gastric tube. Groups IV rats were given magnetic water freely. Group V rats were given 1,2-dimethyl hydrazine + amygdalin. Group VI rat were given 1,2-dimethyl hydrazine + magnetic water Group VII rats were given 1,2-dimethyl hydrazine + amygdalin + magnetic water. Liver histological changes were studied. Degenerative changes were observed in different areas of liver tissue in 1,2-dimethyl hydrazine group, and these changes include: Fibrosis with the appearance of cell necrosis, hemorrhage, fatty infiltration and pleomorphic nuclei. While other groups showed normal appearance of the hepatic cells but some changes were observed in 1,2-dimethyl hydrazine + amygdalin + magnetic water group include: Fibrosis with the appearance of cell necrosis, hemorrhage, fatty infiltration and pleomorphic nuclei but changes in this group were less than in 1,2-dimethyl hydrazine group. In conclusion, the present results suggest that the amygdalin and magnetic water have the potential to ameliorate carcinogen 1,2-dimethyl hydrazine induced hepatotoxicity by antioxidant and antiinflammation activity.
Highlights
1,2 Dimethylhydrazine (DMH) is a potent colon carcinogen, inducing colorectal tumors in experimental animals (1 and 2) and is the most widely used model of chemically induced colon carcinogenesis. 1,2-dimethylhydrazine (DMH) induce oxidative stress to both liver and colon tissues (3)
Active metabolite of DMH is metabolized in liver to form azoxymethane and methylazoxymethanol which is further transported to colon via bile or blood to generate its ultimate carcinogenic metabolite, diazonium ion which elicits an oxidative stress by methylating biomolecules of colonic epithelial cells and leads to promutagenic events as a result of inflammation and tumor promotion (5)
The body consists mainly of water and all living process are strongly dependent on water, which is secret of life that leads to investigate the possibility of improving quality of water by passing it through a magnetic field with different devices and strength
Summary
1,2 Dimethylhydrazine (DMH) is a potent colon carcinogen, inducing colorectal tumors in experimental animals (1 and 2) and is the most widely used model of chemically induced colon carcinogenesis. 1,2-dimethylhydrazine (DMH) induce oxidative stress to both liver and colon tissues (3). Active metabolite of DMH is metabolized in liver to form azoxymethane and methylazoxymethanol which is further transported to colon via bile or blood to generate its ultimate carcinogenic metabolite, diazonium ion which elicits an oxidative stress by methylating biomolecules of colonic epithelial cells and leads to promutagenic events as a result of inflammation and tumor promotion (5). Amygdalin is composed of two molecules of glucose, one of benzaldehyde, which induces an analgesic action, and one of hydrocyanic acid, which is an anti-neoplastic compound It has been used as a traditional drug because of its wide range of medicinal benefits. The body consists mainly of water and all living process are strongly dependent on water, which is secret of life that leads to investigate the possibility of improving quality of water by passing it through a magnetic field with different devices and strength. Some histopathological changes were observed in DMH+Amg+M.W. group include: Fibrosis with the appearance of cell necrosis, hemorrhage, fatty infiltration and pleomorphic nuclei but changes in this group were less than in DMH group (Fig. 4)
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