Ampullary electroreceptors in catfish (Teleostei): temperature dependence of stimulus transduction

Abstract

The response properties of ampullary electroreceptors have been studied in the catfish Ictalurus nebulosus at skin temperatures between 5 and 35 degrees C. A unimodal relationship between spontaneous activity and temperature was obtained. Mean (+/- SEM) peak discharge rate was 57.3 +/- 1.8 impulses s-1 at 25 degrees C; the receptors were active at 5 degrees C (15.0 impulses s-1) and at 35 degrees C (31.5 impulses s-1). There were no dynamic responses to temperature changes in either the warming or cooling direction. The shape of the frequency characteristic depended on temperature: the peak of the gain curve shifted to low frequencies at low temperatures. There was a concomitant change of the phase characteristic: the intersection at zero degree phase angle shifted to higher frequencies with an increase of temperature, thus increasing the lead at lower frequencies and decreasing the lag at higher frequencies. Latency after combined excitatory and inhibitory impulse stimulation was temperature dependent, ranging from 16.4 ms (5 degrees C) to 5.6 ms (35 degrees C). Application of the specific calcium channel blocker menthol (0.2 mM) suppressed spontaneous activity, the effect becoming more prominent at higher temperatures. Sensitivity to sinusoidal electrical stimulation was also impaired, but to a lesser degree and mainly at lower temperatures. We conclude that the filter properties of the receptor organ can be modelled by a band-pass filter in series with a latency, both of which are temperature dependent. These filter properties might be partially based on the activation kinetics of the transduction channels.