Abstract
Carum carvi L. was used traditionally in different populations for many medical complains. The study was aimed to investigate antimicrobial, anti-oxidant activities and cytotoxicty of fixed oil of Carum carvi L. (seeds). The oil was extraction by petroleum ether (60-80°C) using a Soxhlet apparatus. The oil of Carum carvi L. seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the disc diffusion method. The antioxidant activities were conducted via DPPH radical scavenging assay and cytotoxicty using brine shrimp assay. Antimicrobial activity of fixed oil of C. carvi L. dissolved in methanol (1:10), showed high activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (18 & 14 mm). It also showed against Gram positive bacteria (S. aureus & B.subtilis) (14 & 13 mm) and against (C. albicans) (14 mm). The tested anti-oxidant activity gave (18±0.06 RSA %) in comparison to the control of propylgalate (92±0.01 RSA %). In addition cytotoxicity (brine shrimp lethality Bioassay) verified the safety of the examined extract with an IC50 less than 1000μg/ml. This study conducted for essential oil of C. carvi L. seeds proved to have potent activities against antimicrobial activity In-vitro with verified safety evidence for use.
Highlights
Medicinal plants represent a rich source of antimicrobialagents
The oil solution of Carum carvi L. was tested against four standard bacteria species: two Gram-positive bacteria viz., Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains
The oil of Carum carvi L. seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the disc diffusion method
Summary
Medicinal plants represent a rich source of antimicrobialagents. Plants are used medicinally in different countries andare a source of many potent and powerful drugs [1]. Carum carvi L., belonging to the family: Apiaceae, is one of the earliest cultivated herbs in Asia, Africa and Europe In folk medicine, this plantis used as a carminative for stomach disorders, diarrhea, and colic, as well as in veterinary medicine [6]. Abdalaziz et al.: In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L [10]. It can be chewedraw for the almost immediate relief of indigestion and can bemade into infusions. The study was aimed to investigate antimicrobial, anti-oxidant activities and cytotoxicty of fixed oil of Carum carvi L. The study was aimed to investigate antimicrobial, anti-oxidant activities and cytotoxicty of fixed oil of Carum carvi L. (seeds)
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