Abstract
This study aims to evaluate the in vivo efficacy in terms of elasticity, hydration and anti-wrinkle enhancement of a new cosmetic cream formulation: Apollinea anti-ageing facial cream. The latter is O/W (oil/water) emulsion with high volume percentages of snail secretion filtrate and donkey milk serum in its composition. The investigation has been performed on (twelve) healthy subjects: 8 (eight) females, and 4 (four) males, all presenting evident skin wrinkles in typical facial areas (e.g. forehead and eye contour). The subjects had to apply about 2 grams of Apollinea anti-ageing facial cream every day. The efficacy of the new cosmetic formulation was assessed before and after the cosmetic treatment, precisely 2 hours, to assess the short-term efficacy, and 40 days after, this to evaluate the product long-term efficacy. Elasticity enhancements have been tested through measurements of the viscoelastic properties of the skin, whereas the hydration parameter through measurements of the skin and subcutaneous tissues dielectric constant. All the latter measurements have been performed with specific instrumentation, as discussed more in detail in the following. To evaluate the anti-wrinkles efficacy instead, we proceeded with digital camera imaging and image analysis processed through ImageJ software. The new Apollinea facial cream cosmetic formulation, based on donkey milk whey and snail secretion filtrate from Helix Aspersa Muller, showed significant enhancement effects on skin elasticity and hydration as well as an important skin-wrinkles-reduction.
Highlights
Skin ageing is influenced by a number of intrinsic and extrinsic factors, promoting the accumulation, in the cutaneous structure, of physiochemical alterations [1] [2] leading to a progressive loss of structural integrity and physiological functions [3]
This study aims to evaluate the in vivo efficacy in terms of elasticity, hydration and anti-wrinkle enhancement of a new cosmetic cream formulation: Apollinea anti-ageing facial cream
The main ingredients in this new cosmetic formulation “Apollinea facial cream’’ are donkey milk serum, snail secretion filtrate and vegetable lipids, such as sweet almonds oil and Karite butter, all ingredients that strongly contribute to the anti-age efficacy of the final product
Summary
Skin ageing is influenced by a number of intrinsic and extrinsic factors, promoting the accumulation, in the cutaneous structure, of physiochemical alterations [1] [2] leading to a progressive loss of structural integrity and physiological functions [3]. The main ingredients in this new cosmetic formulation “Apollinea facial cream’’ are donkey milk serum, snail secretion filtrate and vegetable lipids, such as sweet almonds oil and Karite butter, all ingredients that strongly contribute to the anti-age efficacy of the final product. Among the latter donkey milk is the most suited for a dermo-cosmetic application given its high percentage of milk serum proteins and other milk compounds, well known for their high degree of uptake and water retention that promotes skin hydration and prevention of epidermis cells degeneration [5] [6] [7]. The capability in calming irritation symptoms [14] [15] and in restructuring skin-ageing processes [16] [17] of Serum-proteins as β-lactoglobulin and lysozyme and essential amino acids beautifying properties are well known [18]
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