Abstract
Plant growth hormone BAP (benzyl amino purine), KIN (kinetin), NAA (1-naphthalene acetic acid) and IBA (indole-3 butyric acid) effect was studied on in vitro multiplication of shoots and rooting of Drepanostachyum falcatum. In vitro micropropagation of himalayan weeping bamboo is explained by in vitro shoot induction and proliferation. Excised explant with axillary bud is surface sterilized with 0.1% HgCl2 for 10 - 12 minutes, cleaned with 90% ethanol and inoculated on liquid Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP/ KIN. Effect of BAP/KIN on shoot induction is with different rate and number of shoots produced by explants with axillary bud cultured on MS media supplemented with 0.0 mg/L BAP/KIN - 5.5 mg/L BAP/KIN. Shoot multiplication with highest rate is achieved on MS medium supplemented with 3.5 mg/L BAP after 4th sub-culturing. The most effective with highest rate and number of root induction combination is 6.5 mg/L IBA after 5 weeks. The roots produced by 6.5 mg/L IBA is best compared with other combination of auxin NAA (1-naphthalene acetic acid).
Highlights
Drepanostachyum falcatum is economically important bamboo commonly known as Himalayan weeping bamboo are the most harvested species for making baskets, mats, flowerpots etc. and other commercial purposes in
The aim of this study is to develop in vitro micropropagation of Drepanostachyum falcatum through tissue culture
The surface sterilized axillary buds were cultured on semi-solid and liquid MS (Murashige and Skoog, 1962) medium supplemented with different concentrations of cytokinin (0.0 - 10.0 mg/l BAP/KIN) [8]
Summary
Drepanostachyum falcatum is economically important bamboo commonly known as Himalayan weeping bamboo are the most harvested species for making baskets, mats, flowerpots etc. and other commercial purposes inHow to cite this paper: Saini, H., Arya, I.D., Arya, S. and Sharma, R. (2016) In Vitro Micropropagation of Himalayan Weeping Bamboo, Drepanostachyum falcatum. It is used in compounding many medicines, making lotion for cleaning wounds [1] and used as soil and water conservation tool [2]. This species is introduced in Nilgiri Hills, Europe, South and North America, Laos and Entebbe Botanic Garden, Uganda. It grows at altitudes up to 2100 m in high slopes, high terraces in moist, sheltered, shady conditions It grows well in sheltered shady spot into a 3 - 4 meter plant with masses of small drooping leaves and prefers rich sandy loam to clayey loam soil often mixed with stones. The importance of this species is highlighted by the diverse uses; it is put to by the locals
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