Abstract
In this study, our objective is to investigate the antioxidant activity by the means of two methods: the β-carotene bleaching method and DPPH assay as well as testing the antibacterial activity by the Agar-well diffusion method, of the extracts (EEp, EDm, EMe and EAq) from the sheets of Hertia cheirifolia. The quantitative analysis are showed that the highest content of total phenolic was concentrated in the methanolic extract with 30.33 + 2.82 μg EAG/mg of extracts, in the second level the EAq extract and EDm extract with 25.92 + 7.19 μg EAG/mg of extracts and 21.25 +1.76 μg EAG/mg of extracts respectively. The content of polyphenols was determined specrophotometrically and showed the presence of these compounds in all extracts. The analysis by TLC revealed the presence of quercetin in the EMe extract of this plant. In the β-carotene bleaching test, the EMe of this plant displayed highest antioxidant activity (72.97%), than in the DPPH assay with a radical-scavenging activity (72.74%). Our results of the antibacterial activity showed the inefficiency of the whole extracts against most of the tested strains.
Highlights
The use of plants as source of remedies for the treatment of many diseases dated back to prehistory; and many populations have this old tradition
Plants used in traditional medicine are still understudied, in clinical microbial ogy
The goals of this study is to evaluate the antioxidant and anti-ridicalizing activity using whitening test of the β-Carotene; and DPPH test; to evaluate the antibacterial activity by the method of diffusion in medium gelose of the rough extracts of Hertia cheirifolia
Summary
The use of plants as source of remedies for the treatment of many diseases dated back to prehistory; and many populations have this old tradition. Despite the remarkable progress in synthetic organic chemistry in the twentieth century, over 25% of prescribed medicines in industrialized countries derived directly or indirectly from plants [1]. Plants used in traditional medicine are still understudied, in clinical microbial ogy. It is reported that most antibiotics derived from microorganisms, and one to three antibiotics are launched every year [2]. Hydroxylated phenolic compounds present in the extractive fraction of several plant materials. Polyphenols in plants include hydroxycoumarins, hydroxylcinnamate derivatives, flavanols, flavonols, flavanones, flavones, anthocyanins, proanthocyanidins (tannins), hydroxystilbenes, aurones, etc. Polyphenols are well documented to have microbicide activities against a huge number of pathogenic bacteria [4]
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