&amp;lt;em&amp;gt;Hedwigia ciliata &amp;lt;/em&amp;gt;(Hedw.) P. Beauv. moss extracts - Chemical characterization and &amp;lt;em&amp;gt;in vitro &amp;lt;/em&amp;gt;testing of immunomodulatory potential

Bojan Božić,Mariana Oalđe,Aneta Sabovljević,Biljana Božić Nedeljković,Marija Mandić,Marko Sabovljević,Tanja Lunić,Sonja Duletić-Laušević,Uroš Gašić

doi:10.3390/ecmc2020-07281

Abstract

Owing to structural diversity and potental pharmacological activity, bioactive compounds derived from natural sources are in the focus of intensive exploration. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. was examined, and further tested for antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative activity in vitro. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical analysis revealed the presence of phenolics, flavonoids, and triterpenoids as secondary metabolites of high biological activity. Antioxidant activity of extracts was assessed using three different methods, DPPH (2,2-diphenyl-1-picrylhydrazyl), total reducing power assay, and β-carotene/linoleic acid assay. To evaluate the enzyme-inhibitory activity, extracts were screened for α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase inhibitory effects. The biocompatibility of moss extracts was confirmed using normal human fibroblast cell line, MRC-5. The H. ciliata extracts displayed significant antiproliferative activity (~ 50% inhibition) against the MDA-MB-231 (human breast adenocarcinoma) cells, which had not previously been reported elsewhere. The potential anti-neuroinflammatory activity was observed due to significant reduction of the NO production by LPS-stimulated BV2 (normal murine microglia cell line), being in line with previously recognized antineurodegenerative potential, as measured by the inhibition of acetylcholinesterase and tyrosinase activity. Furthermore, the H. ciliata extracts displayed significant antidiabetic effect via α-glucosidase inhibition. In general, the obtained results suggest the potential immunomodulatory activity of the moss H. ciliata extracts, remaining to be characterized in more detail in search for mechanisms underlying the immunomodulation processes.

Highlights

As a result of an excessive inflammatory response, various acute and chronic diseases can occur, as well as tumors
Owing to structural diversity and potental pharmacological activity, bioactive compounds derived from natural sources are in the focus of intensive exploration
The increased rate of tumor diseases has led to an expanding number of studies aimed at prevention, diagnosis, prognosis and therapy

Summary

Introduction

As a result of an excessive inflammatory response, various acute and chronic diseases can occur, as well as tumors. The increased rate of tumor diseases has led to an expanding number of studies aimed at prevention, diagnosis, prognosis and therapy. Some of the currently identified moss secondary metabolites belong to phenolics, flavonoids and terpenoids which are considered to have antioxidative, antiproliferative and anti-inflammatory properties. Mosses represent a large group of plants that are widespread in almost every part of the world. One of the oldest plants on the planet there are about 20,000 species which include: 1. Mosses have been used in traditional medicine since ancient times for the treatment of various pathological conditions

Results and discussion

Findings

Conclusions