Abstract
AFLP (Amplified Fragment Length Polymorphism) markers were investigated for assessing diversity and fingerprinting of a selected sample of soft red winter wheat (SRWW) genotypes that differed for pre-harvest sprouting (PHS) resistance and for the presence of awns. Sixty AFLP primer pairs generated 2944 polymorphic bands out of a total of 6778 bands scored among 15 SRWW genotypes. The mean polymorphism information content was 0.37 and the average marker index was 15.1, indicating that AFLP generated more information in wheat than other molecular markers. Forty-two primer pairs that contained no missing data points were used to create a dendrogram to estimate the genetic distance among genotypes. A total of 4761 loci, of which 1915 were polymorphic, were included in the genetic similarity matrix. Primer pairs showed a high degree of correlation between four arbitrarily divided primer subsets (r=0.82). The SRWW genotypes investigated did not group together regarding resistance to PHS or the presence of awns. The mean genetic similarity values among the 15 genotypes ranged from 0.83 to 0.94. Although the genetic similarity among these soft red winter wheat genotypes was high, differences were found that could be used for assessing genetic diversity as well as for plant variety protection use.
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