Abstract
Polymerase chain reaction (PCR) was performed to detect genomic differences between healthy and paulownia witches'-broom (PaWB)-affected paulownia plants using fifteen primer-pair combinations derived from five randomly synthesized primers based on the P1-like adhesin gene sequence of Mycoplasma pneumoniae. Upon comparing the PCR product profiles between healthy and PaWB-affected paulownia DNA samples, three types of PCR profiles were observed. Firstly, some bands were amplified from both healthy and PaWB-affected paulownia DNA samples; secondly, some DNA bands were amplified only from PaWB-affected paulownia DNA samples; thirdly, a few DNA bands were amplified only from healthy paulownia DNA samples. DNA amplified from PaWB-affected paulownia DNA samples hybridized only with PaWB-affected paulownia DNA samples, suggesting the DNA was derived from paulownia phytoplasma genome.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.