Amplification of Antioxidant Activity of Haptoglobin(2-2)–Hemoglobin at Pathologic Temperature and Presence of Antibiotics

Abstract

It is clear that Haptoglobin binds to Hemoglobin strongly and irreversibly. This binding, protects body tissues against heme-mediated oxidative tissue damages via peroxidase activity of Haptoglobin-Hemoglobin complex. Peroxidase activity of Haptoglobin(2-2)-Hemoglobin complex was determined via measurement of following increase in absorption of produced tetraguaiacol as the second substrate of Haptoglobin-Hemoglobin complex by UV-Vis spectrophotometer at 470nm and 42°C. The results are showing that peroxidase activity of Haptoglobin(2-2)-Hemoglobin complex is modulated by homotropic effect of hydrogen peroxide as the allosteric substrate. On the other hand, antioxidant activity of Haptoglobin(2-2)-Hemoglobin is increased via heterotropic effect of two antibiotics (especially ampicillin) on the peroxidase activity of the complex. The condition of pathologic temperature along with the administration of ampicillin and/or coamoxiclav is in favor of amplification in antioxidant activity of Haptoglobin(2-2)-Hemoglobin and combating against free radicals in individuals with Hp2-2 phenotype. Therefore, oxidative stress effects have been diminished in the population with this phenotype.