Ampicillin enhanced the resistance of Myzus persicae to imidacloprid and cyantraniliprole.

Abstract

Recent studies have shown that symbionts are involved in regulating insecticide detoxification in insects. However, there are few studies on the relationship between the symbionts found in Myzus persicae and the mechanism underlying host detoxification of insecticides. In this study, antibiotic ampicillin treatment was used to investigate the possible relationship between symbiotic bacteria and the detoxification of insecticides in the host, M. persicae. Bioassays showed that ampicillin significantly reduced the susceptibilities of M. persicae to imidacloprid and cyantraniliprole. Synergistic bioassays and RNAi assays showed that the susceptibilities of M. persicae to imidacloprid and cyantraniliprole were related to metabolic detoxification enzyme activities and the expression level of the cytochrome P450 gene, CYP6CY3. Also, treatment to a combination of ampicillin and enzyme inhibitors or dsCYP6CY3 showed that the negative effect of ampicillin on the susceptibility of M. persicae was effectively inhibited bydetoxification enzyme inhibitors and dsCYP6CY3. Additionally, ampicillin treatment resulted in significant increases in the activities of multifunctional oxidases and esterases, the expression level of CYP6CY3 and fitness of M. persicae. Further, ampicillin significantly reduced the total bacterial abundance and changed symbiont diversity in M. persicae. The abundance of Pseudomonadaceae decreased significantly, while the abundance of Rhodococcus and Buchnera increased significantly. Our study showed that ampicillin enhanced the resistance levels to imidacloprid and cyantraniliprole of M. persicae, which might be related to the selective elimination of symbiotic bacteria, the upregulated activities of detoxification enzymes and the increased fitness. © 2022 Society of Chemical Industry.