Abstract
Fragments of pancreas from the adult urodele amphibian Amphiuma means (the Congo eel) were maintained in organ culture for up to 28 days. Both amylase synthesis and release into the culture medium were stimulated by pancreozymin. Amylase release was also stimulated by the addition to the medium of dibutyryl-cAMP, acetylcholine, methacholine, glucagon, isoprenaline, calcium, and an ionophore. The pancreozymin effect was inhibited by dinitrophenol, that of acetylcholine by atropine. Added glucose, insulin, and a protease inhibitor did not affect medium amylase levels. The results are discussed in terms of long-term adult vertebrate pancreas organ culture and the comparative control of amylase release by the exocrine pancreas.
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