Amphibian acid phosphatase—II.: The effect of parathyroid extract on the lysosomal enzyme, acid phosphatase, in tissues of Rana pipiens

Abstract

1. 1. Acid phosphatase activity is influenced by parathyroid hormone in amphibian skeletal tissues and plasma. 2. 2. The enzymatic response to parathormone is tissue-specific, varying in time, magnitude and direction of change. 3. 3. The diaphysis and epiphysis of the femur respond slowly to parathyroid extract treatment; at 1 hr after injection, acid phosphatase activities were little changed from control levels, but subsequently, a progressive increase was observed. Plasma enzyme activity rises through 4 hr. 4. 4. The metaphyseal plate of the femur and the spongy coracoid bone of the pectoral girdle respond rapidly and strongly to hormone treatment; from 1–4 hr after injection, their acid phosphatase activities are significantly elevated. 5. 5. Daily hormone treatment for 1 week elevates acid phosphatase activity in plasma and all skeletal tissues studied except femoral epiphysis. On day 8, enzyme activity of the latter had sharply declined. 6. 6. A significant hypercalcemia accompanies the enzymatic changes in skeletal tissues and plasma.