Abstract
A novel tyrosinase amperometric biosensor based on boron-doped nanocrystalline diamond (BDND) film electrode has been developed for the detection of phenolic compounds. Amine-terminated BDND surface was achieved via a direct photochemical reaction with allylamine. The tyrosinase was then covalently immobilized on the amine-terminated diamond film electrode surface. The effects of pH and applied potential on the performance of tyrosinase amperometric biosensor were investigated. The biosensor showed an optimum response at a pH of 6.5 and at an applied potential of −100 mV. It also exhibited good analytical performances to phenolic compounds in terms of sensitivity, detection limit, and stability. The tyrosinase-modified BDND film electrode exhibited a rapid response to the changes in the substrate concentration for all the phenolic compounds tested and reached 95 % of steady-state current in about 10 s. It is attributed to the high loading of tyrosinase and the rapid electron transfer between the tyrosinase and BDND film electrode surface. The tyrosinase-modified BDND film electrode showed high sensitivity with 184.0, 95.6, and 552.3 mA M−1 cm−2 and a linear response range of 10.0–120.0, 5.0–120.0, and 30.0–120.0 μM for phenol, catechol, and 4-chlorophenol, respectively.
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