Abstract
An amperometric biosensor for adenosine-5‘- triphosphate (ATP) was developed applying a competitive assay of two glucose converting enzymes. Hexokinase and pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase were entrapped by pH-shift induced deposition of Resydrol at a platinum microelectrode with a diameter of 25 μm. Different mediators for the PQQ-dependent glucose deydrogenase (GDH) catalyzed reaction were used applying potentials not higher than 400 mV vs. Ag/AgCl reference electrode. The obtained biosensor is selective to ATP and sensitive to it at physiological concentrations down to the nanomolar range.
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