Abstract
An amperometric electrode sensitive to pyruvate based on methylene green as mediator and on carbon paste chemically modified with pyruvate oxidase is described. The electrode generates an anodic current in the range 0.0-0.5 V vs. SCE. The optimum pH is 7.0. The apparent Michaelis constant, K m(app), for the calibration graphs for the electrode depends on the amount of pyruvate oxidase and changes in the range 1.2–1.6 mM at a phosphate concentration of 0.1 M. The electrode sensitivity achieved is 51.8 μA 1 mmol −1 cm −2 and does not depend on the normal oxygen concentration in solution. The electrode responds to inorganic phosphate, and K m(app) was 87 mM at a pyruvate concentration of 2.1 mM. The electrodes were used for the determination of pyruvate in human sweat. The electrodes remained stable for more than 2 months.
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