Abstract
A non-conducting polymer film of phenol and its derivatives, in which glucose oxidase (GOx) was immobilized, has been prepared on a carbon paste electrode containing ferrocene by electrochemical polymerization. Five phenol derivatives (phenol, 3-aminophenol, 3-methylphenol, 3-nitrophenol, and 1,3-dihydroxybenzene) were examined; 3-aminophenol was found to be the most suitable monomer for the fabrication of amperometric glucose sensors. The maximum current Δimax obtained from the Eadie-Hofstee form of the Michaelis-Menten equation decreased with increasing the substituent constant σm; a plot of Δimaxvs. σm gave a straight line except for 3-aminophenol. In the case of 3-aminophenol, both hydroxyl and amino groups participated in the electropolymerization and consequently larger amounts of GOx may be immobilized in this polymer film. This behavior resulted in the higher sensitivity to glucose. The response currents of Fc,CP/GOx/PAPO, which was fabricated by cycling between 0.20 and 1.40 V vs. Ag/AgCl for the electropolymerization of 3-aminophenol, changed little within a period of 40 d. In addition, this glucose sensor could minimize contributions from the easily oxidizable compounds, uric acid and L-ascorbic acid.
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