Amperometric Glucose Enzyme Sensor Prepared by Immobilizing Glucose Oxidase on CuPtCl6 Chemically Modified Electrode

Abstract

A simple, sensitive, stable and low-cost glucose sensor with a fast response that operates under aerobic solution conditions is described. The electrode consisted of a CuPtCl6 modified film deposited on the surface of the glassy carbon electrode onto which the glucose oxidase was immobilized via cross-linking of glutaraldehyde in the presence of bovine serum albumin (BSA). The electropolymerized CuPtCl6 film was prepared by continuously cyclically scanning between 700 mV and –800 mV (vs. SCE) in a solution containing CuCl2 and K2PtCl6. The preparation and the performance of the enzyme electrode have been discussed. The effects of enzyme loading, pH, and the temperature have been studied. The experiments on interference and specificity have also been demonstrated. The enzyme electrode exhibits an excellent biocatalytic activity and stability. The enzyme electrode provides a linear response to glucose over a concentration range of 2×10–6 M to 1×10–3 M with a detection limit of 1×10–6 M.