Abstract
A novel and robust amperometric enzyme electrode for the determination of glucose was constructed by immobilizing glucose oxidase (GOD) and Os(bpy) 2Cl–poly(4-vinyl)pyridine (Os–PVP) complex multilayers on thiol self-assembled monolayers surface. The apparent Michaelis-Menton constant K m′ increased with increasing the number of Os–PVP/GOD multilayers. The concentration range of linear response and detection limit were 0.1–10 and 0.05 mM, the interference of ascorbic acid and uric acid were eliminated by the presence of SAMs and the enzyme electrodes were stable over 3 weeks. The preparation technique may be useful for controlling the performance of multilayer enzyme electrodes by changing the enzyme content.
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