Abstract
An amperometric organic-phase peroxide biosensor was constructed by immobilizing horseradish peroxidase (HRP) entrapped within poly(ester-sulfonic acid) Eastman AQ-55D polymer matrix on a platinum electrode surface. The enzyme electrode functioned by direct electron transfer between the HRP redox centre and the platinum surface. This mediator-free biosensor was applied to the determination of butanone peroxide and hydroxylamine, which are HRP substrate and inhibitor, respectively. Fast sensor responses were obtained in 30 s for both the substrate and the inhibitor. The biosensor showed higher catalytic efficiency in acetonitrile (1.1 µA l mmol–1) than in methanol (0.9 µA l mmol–1). However, the sensor exhibited greater percentage inhibition of HRP activity in the less catalytically efficient methanol medium (516% inhibition 1 mmol–1) than in acetonitrile (345% inhibition l mmol–1). This reversal in the performance of the sensor towards hydroxylamine in the organic solvents is attributed to the reaction media polarity combined with the butanone peroxide-induced conformational change of the enzyme active site.
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More From: Analytical Proceedings including Analytical Communications
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