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Abstract
A reagentless H2O2 sensor based on the direct electron transfer of myoglobin (Mb) doped in multiwalled carbon nanotubes enhanced grafted collagen matrix is proposed. The formal potential of the immobilized Mb was −0.358 V with a surface coverage of 4.0×10−10 mol cm−2. The electrode process was surface‐controlled with an electron transfer rate constant of 9.7 s−1. The proposed biosensor displayed an excellent electrocatalytic response to the reduction of H2O2 with a linear range from 0.6 to 39.0 µM. Owing to the good biocompatibility and high enzyme loading of the matrix the biosensor exhibited acceptable stability and reproducibility.
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