Abstract

A urea biosensor based on the amperometric determination of ammonia produced by hydrolytic decomposition of urea by urease is described. The urease is immobilized on the amperometric ammonium ion-selective electrode sensor. This urea sensor gives the current response proportional to the concentration of urea in the range of a few to 100 ΩM in test solutions. The response can be corrected for the residual ammonium ions. The urea sensor was successfully applied to the analysis of biological fluids. An amperometric creatinine biosensor is also described.

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