AmpC β-Lactamase Variable Expression in Common Multidrug-Resistant Nosocomial Bacterial Pathogens from a Tertiary Hospital in Cairo, Egypt.

Aliaa Ali El Shamy,Hebatallah A Nasser,Al-Taher Rabea,Mahmoud M Tolba,Mahmoud M Tawfick,Zainab Zakaria,Nermeen Salah Eldin

doi:10.1155/2021/6633888

Abstract

The emergence of AmpC (pAmpC) β-lactamases conferring resistance to the third-generation cephalosporins has become a major clinical concern worldwide. In this study, we aimed to evaluate the expression of AmpC β-lactamase encoding gene among the pathogenic Gram-positive and Gram-negative resistant bacteria screened from clinical samples of Egyptian patients enrolled into El-Qasr El-Ainy Tertiary Hospital in Cairo, Egypt. A total of 153 bacterial isolates of the species Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterococcus faecium were isolated from patients diagnosed with urinary tract infection (UTI), respiratory tract infection (RTI), and wound infections. The total number of E. faecium isolates was 53, comprising 29 urine isolates, 5 sputum isolates, and 19 wound swab isolates, whereas the total number of P. aeruginosa isolates was 49, comprising 27 urine isolates, 7 sputum isolates, and 15 wound swab isolates, and that of the K. pneumoniae isolates was 51, comprising 20 urine isolates, 25 sputum isolates, and 6 wound swab isolates. Our results indicated that there is no significant difference in the expression of AmpC β-lactamase gene among the tested bacterial species with respect to the type of infection and/or clinical specimen. However, the expression patterns of AmpC β-lactamase gene markedly differed according to the antibacterial resistance characteristics of the tested isolates.

Highlights

Aliaa Ali El Shamy,1 Zainab Zakaria,2 Mahmoud M
A total of 153 bacterial isolates of the species Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterococcus faecium were isolated from patients diagnosed with urinary tract infection (UTI), respiratory tract infection (RTI), and wound infections. e total number of E. faecium isolates was 53, comprising 29 urine isolates, 5 sputum isolates, and 19 wound swab isolates, whereas the total number of P. aeruginosa isolates was 49, comprising 27 urine isolates, 7 sputum isolates, and 15 wound swab isolates, and that of the K. pneumoniae isolates was 51, comprising 20 urine isolates, 25 sputum isolates, and 6 wound swab isolates
Klebsiella pneumoniae is a type of Gram-negative bacteria associated with a wide spectrum of infections, such as UTI, pneumonia, intra-abdominal infections, bloodstream infections (BSI), meningitis, and pyogenic liver abscesses (PLA) [7], whereas Enterococcus faecium is a Gram-positive bacterium that began to emerge as a leading cause of multidrug-resistant hospitalacquired infections in the 1970s and 1980s, and it currently ranks among the leading causes of hospital-acquired infections of the bloodstream, urinary tract, surgical wounds, and other sites

Summary

Introduction

Aliaa Ali El Shamy ,1 Zainab Zakaria, Mahmoud M. E widespread and incautious use of antibiotics has significantly contributed to the advent of resistant microbial strains [2] Resistance to antimicrobials such as the third-generation cephalosporins, quinolones, and carbapenems continues, to increase sharply among Gram-negative bacterial pathogens [3]. Klebsiella pneumoniae is a type of Gram-negative bacteria associated with a wide spectrum of infections, such as UTI, pneumonia, intra-abdominal infections, BSI, meningitis, and pyogenic liver abscesses (PLA) [7], whereas Enterococcus faecium is a Gram-positive bacterium that began to emerge as a leading cause of multidrug-resistant hospitalacquired infections in the 1970s and 1980s, and it currently ranks among the leading causes of hospital-acquired infections of the bloodstream, urinary tract, surgical wounds, and other sites. It is found to accompany the obligate anaerobes in mixed infections which results in intra-abdominal abscesses [8]

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