Abstract
Background: The plasminogen activator inhibitor-1 (PAI-1) is a puissant antifibrinolytic factor; plasma PAI-1 level is high in type 2 diabetes. 4G/5G polymorphism of PAI-1 gene is a major genetic determinant of plasma PAI-1 levels, with 4G carriers having high PAI-1 level than 5G, theses pose the question about relation T2 patients and those polymorphisms. The aim of this study was to determine the relationship between the polymorphisms −675 4G/5G and −844 G/A of PAI-1 gene and type 2 diabetes mellitus. Methods: A case control study of 491 diabetic and 400 healthy controls. Genotyping of the polymorphism −675 4G/5G was done by PCR-ASA (polymerase chain reaction, allele specific amplification), and the polymorphism −844 G/A was done with PCR-RFLP (restriction fragment length polymorphism), the allelic frequency is calculated with hardy-Weinberg law, the statistic analysis was done by SPSS version 10. Results: Higher frequencies of The genotypes 4G/4G (p = 0.01) and 4G/5G of polymorphism −675 4G/5G were seen in diabetic (p = 0.05) and higher frequencies of 5G/5G was seen in controls (p −844 G/A was seen in diabetics and G/G was seen in controls (p = 0.01). Conclusion: Our study found association between 4G allele of −675 4G/5G and A allele of −844 G/A of PAI-1 gene and having type 2 diabetes mellitus in Tunisian population.
Highlights
plasminogen activator inhibitor-1 (PAI-1) is a powerful inhibitor of fibrinolysis
The plasminogen activator inhibitor-1 (PAI-1) is a puissant antifibrinolytic factor; plasma PAI-1 level is high in type 2 diabetes. 4G/5G polymorphism of PAI-1 gene is a major genetic determinant of plasma PAI-1 levels, with 4G carriers having high PAI-1 level than 5G, theses pose the question about relation T2 patients and those polymorphisms
Genotyping of the polymorphism −675 4G/5G was done by PCR-ASA, and the polymorphism −844 G/A was done with PCR-RFLP, the allelic frequency is calculated with hardy-Weinberg law, the statistic analysis was done by SPSS version 10
Summary
PAI-1 (plasminogen activator-inhibitor-1) is a powerful inhibitor of fibrinolysis. So it is a thrombogenic factor, it is incriminated in atherosclerosis [1] and vein thromboses or are high rate [2]. Type 2 diabetes is a disease with a very high cardiovascular risk [3] [4], where the level of PAI-1 Ag is increased [5] This plasma level is determined by genetic polymorphisms [6] and environmental factors [7]. The plasminogen activator inhibitor-1 (PAI-1) is a puissant antifibrinolytic factor; plasma PAI-1 level is high in type 2 diabetes. The aim of this study was to determine the relationship between the polymorphisms −675 4G/5G and −844 G/A of PAI-1 gene and type 2 diabetes mellitus. Conclusion: Our study found association between 4G allele of −675 4G/5G and A allele of −844 G/A of PAI-1 gene and having type 2 diabetes mellitus in Tunisian population
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
