Abstract
Plantlets of Curcuma caesia were produced in vitro from newly sprouting vegetative buds of tubers. Segments of the plantlets from the junction between the root and the basal portion of the stem were subsequently used as explants to investigate factors affecting callus induction and plant regeneration via somatic embryogenesis. The explants were placed on Woody Plant Medium (WPM) together with different concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and benzyl aminopurine (BAP) in the presence of light. The growth medium supplemented with 5 mg/L BAP and 2 mg/L 2,4-D promoted callus induction after 70 days of culture. Sub-culturing on the same medium enhanced the production of friable callus. Culture media containing higher concentrations of agar promoted the development of green somatic embryos from the callus. Respond of somatic embryogenesis was most successful with MS medium in 6.0 g/L agar supplemented with 5 mg/L BAP and 0.2 mg/L 2,4-D whereby the callus developed into green somatic embryos with an efficiency of 53%. This culture medium also produced the largest number plantlets.
Highlights
Curcuma caesia L., known as black turmeric, is a perennial herb belonging to the family Zingiberaceae
How to cite this paper: Zuraida, A.R., et al (2014) In Vitro Regeneration of Curcuma caesia Plantlets from Basal Part and via Somatic Embryogenesis
In vitro production of many plant species can be achieved either through direct shoot multiplication or via somatic embryogenesis, neither technique has been published for C. caesia
Summary
Curcuma caesia L., known as black turmeric, is a perennial herb belonging to the family Zingiberaceae. The. How to cite this paper: Zuraida, A.R., et al (2014) In Vitro Regeneration of Curcuma caesia Plantlets from Basal Part and via Somatic Embryogenesis. In vitro production of many plant species can be achieved either through direct shoot multiplication or via somatic embryogenesis, neither technique has been published for C. caesia. According to [5], some of the advantages somatic embryogenesis has over direct in vitro propagation systems include the former’s high multiplication rates, possibility of cryopreservation of embryogenic callus, the potential for scale-up in liquid suspension cultures, amenability to the use of bioreactors and the application of somatic synthetic seed technologies. We describe the in vitro regeneration of C. caesia plantlets through callus production and somatic embryogenesis
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