<i>In vitro</i> Propagation of <i>Dendrobium jerdonianum Wight</i> Through Flower Stalk, Leaf, Nodes/ Internodes as Explants

Abstract

<i>Dendrobium jerdonianum Wight</i> is an Epiphytic tufted orchid at higher elevations. The maximum percentage establishment of Flower stalk, leaf, node and internode explants was observed on MS, VW and KC Medium. However, MS media fortified with activated charcoal exhibited maximum multiplication rate for <i> in vitro</i> rooting. MS, VW and KC media supplemented with various concentrations of auxins and cytokinins were used in flower stalk, leaf, nodes and internodes for plantlet formation. In the evaluation of the media MS basal medium fortified with 0.5mg 2, 4, D-dichloro phenoxy acetic acid +5mg BAP + 50mlsuitable for flower stalk culture. KC medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid was found to be suitable for Leaf and internodes Culture. VW medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 3mg BAP + 50ml CM, was found to be suitable for nodal segments. The entire above medium used with different composition gives highest percentage of 80-95 percent results for plantlet formation. <i> In vitro</i> rooting was successful with MS medium supplemented with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 5mg IAA + 50ml CM and 500mg of activated charcoal. 90 days old <i> in vitro</i> plantlets were hardened and transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here.