Abstract
A protocol for rapid in vitro propagation from nodal explants of the medicinal tree species Aegle marmelos (L.) corr. of family Rutaceae has been described. High frequency bud break were induced on Murashige and Skoog’s (1962) basal medium supplemented with 0.5 mg benzyladenine (BA)/l. After 10 days of culture, nodal explants with multiplied buds started callusing with restricted growth and defoliation. When the same nodal explants ware transferred into the same basal medium supplemented with 0.5 mg BA/l with different concentrations of either kinetin (KN) or gibberellic acid (GA3) or in combinations has shown healthy shoots with expanded shoot length. Excised shoots (2cm-3cm long with 2 to 3 nodes) when grown on 1/2 MS basal medium with 2.5 mg/l indole-3-butyric acid (IBA) and 0.5% activated charcoal (A.C.)/l has shown rhizogenesis. After excision, in the second passage, the nodal explants also showed bud break when sub cultured on MS basal medium supplemented with 0.5 mg BA/l. These shoots also successfully rooted on the same above said medium.
Highlights
Aegle marmelos (L.) corr. is a forest cosmopolitan as well as an important medicinal plant
High frequency bud break were induced on Murashige and Skoog’s (1962) basal medium supplemented with 0.5 mg benzyladenine (BA)/l
When the same nodal explants ware transferred into the same basal medium supplemented with 0.5 mg BA/l with different concentrations of either kinetin (KN) or gibberellic acid (GA3) or in combinations has shown healthy shoots with expanded shoot length
Summary
Aegle marmelos (L.) corr. (family-Rutaceae) is a forest cosmopolitan as well as an important medicinal plant. In recent years there has been an increased interest in in vitro culture techniques which offer a viable tool for mass multiplication and germplasm conservation of rare, endangered, aromatic and medicinal plants [5,6,7,8], as well as forest trees where the lifecycle takes a longer period [9,10]. There are techniques have been reported for in vitro propagation of Aegle marmelos from in vitro grown seedling parts, i.e. from roots [17], from hypocotyl [18], cotyledonary node [19] and excised leaf explants [20] but the works have more been concentrated on in vitro grown seedling explants. In the present study trial has been taken to establish a protocol for regeneration and mass propagation of A. marmelos from mature tree explants i.e. from nodal explants through axillary bud multiplication
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