Abstract
In vitro seeds germination and plantlets establishment of Cladanthus mixtus (L.) Oberpr. and Vogt were studied in this report. A reliable protocol was developed for in vitro seed germination, multiplication and plantlet regeneration of Cladanthus mixtus. The seeds were sterilized and cultured in different media. Among the two basal media evaluated for symbiotic seed germination, gelled distilled water was found to be the best with a high percentage of seeds germination (100%) after 33 hours of culture. Seedlings were further transferred to different types of media. Result observed after 4 weeks showed that MS medium promotes the highest growth with an average of 2.75 ± 0.12 cm shoot length and 2.60 ± 0.29 shoots per explants, and the mean number of roots achieved 3.33 ± 0.17 root per explants with a length of 2.42 ± 0.16 cm. This study showed that macroelements of MS (1962) medium is essential for in vitro shoot multiplication, growth and rooting of shoots of Cladanthus mixtus L.
Highlights
Tribe Anthémidées includes 109 genus and 1740 species [1]
A molecular study of Chamaemelum Mill. and related genus showed that traditionally marked as Chamaemelum is paraphyletic [2]
The aim of this paper is to test the effect of different culture media on in vitro seed germination of Cladanthus mixtus (L.), and micropropagation, multiplication and rooting of seedling germination
Summary
Tribe Anthémidées includes 109 genus and 1740 species [1]. The phylogenetic studies have confused the namesHow to cite this paper: Harras, N. and Lamarti, A. (2014) In Vitro Germination and Plantlet Establishment of Wild Chamomile of Morocco Cladanthus mixtus (L.) Oberpr. and Vogt. Tribe Anthémidées includes 109 genus and 1740 species [1]. The phylogenetic studies have confused the names. How to cite this paper: Harras, N. and Lamarti, A. (2014) In Vitro Germination and Plantlet Establishment of Wild Chamomile of Morocco Cladanthus mixtus (L.) Oberpr. American Journal of Plant Sciences, 5, 2623-2632. Lamarti of species Anthemideae, including genus Chamaemelum, Ormenis and Cladanthus [2]. Genus Cladanthus is clearly related to the genera Anthemis and Chamaemelum and most species were previously included in the last genus. Related genus showed that traditionally marked as Chamaemelum is paraphyletic [2] A molecular study of Chamaemelum Mill. and related genus showed that traditionally marked as Chamaemelum is paraphyletic [2]
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