Abstract
Viral load quantification is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate biomarker of AIDS. High viral load indicates that the immune system is failed to fight against viruses. The aim of this study was to evaluate the impact of biofield treatment on HIV-1 and HCMV in terms of viral loads as surrogate marker. The viral load assay was performed on stored stock cultures of HIV infected human plasma samples before and after 7 days of biofield treatment using Roche COBAS® AMPLICOR analyzer. Viral load (HIV-1 RNA and HCMV DNAaemia) was considered as surrogate marker for assessment of the impact of Mr. Trivedi’s biofield treatment in HIV infected stored plasma samples. The viral load quantification of HIV-1 RNA in infected stored plasma samples was significantly reduced by 65% in biofield treated group as compared to control. Additionally, viral load of HCMV DNAaemia in infected stored plasma samples was also reduced by 80% in the biofield treated group as compared to control. Because, children are more prone to HCMV infection and adults are generally liable to suffer from HIV-1 infection. As the biofield treatment has reduced HCMV DNAaemia, it could be beneficial for HIV infected children populations. Altogether, data suggest that biofield treatment has significantly reduced the viral load quantification in HIV-1 and HCMV infected stored plasma samples and could be a suitable alternative treatment strategy for AIDS patients in near future.
Highlights
Human immunodeficiency virus type 1 (HIV-1) is the main causative agent of acquired immune deficiency syndrome (AIDS) [1]
Two sets of each viral samples (HIV-1; 31 samples and Human cytomegalovirus (HCMV); 5 samples) of HIV and HCMV infected stored plasma were used in this experiment for determination of viral load quantification
Higher the viral titer indicates that the immune system is failed to fight against HIV or HCMV
Summary
Human immunodeficiency virus type 1 (HIV-1) is the main causative agent of acquired immune deficiency syndrome (AIDS) [1]. Human cytomegalovirus (HCMV) is a vernacular name of the human herpes virus - 5, a highly host-specific virus of the herpesviridae family rarely causes symptoms. The pregnant women and immune weakened persons are highly prone to acquire infection by HCMV virus. It spreads through various body fluids, such as blood, urine, saliva, semen, and breast milk. It may causes serious morbidity and mortality in organ transplant recipients, immunocompromised, HIV infected patients and congenitally infected newborns [4]. The viral ‘threshold load’ is very sensitive and specific for predicting
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