Abstract
This study aimed to describe the factors associated with biofilms formation in dental pathology by comparison of bacterial growth on dental and stainless-steel surfaces. We studied in vitro the behavior of Staphylococcus aureus Metis in order to observe the capacity of adhesion, to evaluate quantitatively the potential of proliferation and to compare the behavior of this germ in contact with the two surfaces. The biomaterials used were cylinders in Stainless steel (AISI 316L), dental fragments and stainless-steel fragments, all were disinfected for 15 minutes and then sterilized in a wet autoclave at 120˚C for 30 min. Macroscopic observation with a binocular magnifier of bacterial proliferation was carried out regularly after 6 h and 24 h of incubation. Observation by optical microscope based on GRAM staining made it possible to visualize the presence or absence of bacteria and to differentiate them. The adhesion of Staphylococcus aureus Meti S on dental fragments was compared to the one obtained on stainless steel fragments. We also carried a Bacterial count by optical dosing. The results show that the ability of this germ to colonize and develop biofilms on surfaces depends mainly on the characteristics of the surface. Rough surfaces as dental surface are more likely to developing biofilms than smooth surfaces like stainless-steel surface.
Highlights
The oral cavity is home to one of the most complex bacterial ecosystems in the body
The aim of this work was to study in vitro the behavior of certain bacteria of the oral flora in contact with a dental surface and stainless steel to: - Observe the ability of these germs to adhere to dental surfaces and to stainless steel surfaces. - Quantitatively assess the adhesion and proliferation potential of these germs in contact with dental surfaces and titanium. - Compare their behavior on contact with these 2 surfaces
- After 6 hours of incubation: (Figure 3) No bacterial culture was observed on the plates encrusted with dental fragments and on the stainless steel fragments. - After 24 hours of incubation: (Figure 4)
Summary
The oral cavity is home to one of the most complex bacterial ecosystems in the body. This natural cavity, along with the colon, is the most septic part of the human body. Many authors have tried to quantify it: a milligram of plaque contains approximately 100 million bacteria; 1 milliliter of saliva contains an average number of 750 million bacteria (including 100 million bacteria that can be cultivated on culture medium) [1]. These metallic biomaterials are exposed to many factors such as saliva, bacterial microflora, food, temperature fluctuations, and mechanical forces. Leading to ecological changes in the oral environment and increase of Streptococcus mutans count in the saliva and dental plaque [2]
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