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Abstract
In recent years, Jatropha curcas L. has gained popularity as a potential biodiesel plant. The varying oil content, reported between accessions belonging to different agroclimatic zones, has necessitated the assessment of the existing genetic variability to generate reliable molecular markers for selection of high oil yielding variety. EST derived SSR markers are more useful than genomic markers as they represent the transcriptome, thus, directly linked to functional genes. The present report describes the in silico mining of the microsatellites (SSRs) using J. curcas ESTs from various tissues viz. embryo, root, leaf and seed available in the public domain of NCBI. A total of 13,513 ESTs were downloaded. From these ESTs, 7552 unigenes were obtained and 395 SSRs were generated from 377 SSR-ESTs. These EST-SSRs can be used as potential microsatellite markers for diversity analysis, MAS etc. Since the Jatropha genes carrying SSRs have been identified in this study, thus, EST-SSRs directly linked to genes will be useful for developing trait linked markers.
Highlights
In recent years, Jatropha curcas L. has gained popularity as a potential biodiesel plant
As reported by Raji and coworkers [18], these unigenes, when used for the mining of SSRs result in a realistic estimate of the microsatellite repeat frequency and ensures that non redundant Expressed Sequence Tags (ESTs)-SSR markers that correspond to unique loci in the genome are obtained
It can be said that as the class of the SSR motif size increases, like tetra, pentaand hexa-nucleotide, higher number, rather 100% of microsatellites were found in the category of
Summary
Jatropha curcas L. has gained popularity as a potential biodiesel plant. This plant belongs to the family Euphorbiaceae and is a native of Mexico and Central America and was later on introduced in many parts of tropics and subtropics. J. curcas is commonly known to be a poisonous plant It is a semi-evergreen shrub or small tree reaching a height of 6 mt (20 ft). It can survive arid conditions; can be grown on drylands and wastelands. Being traditionally considered as a weed, its oil has recently started gaining importance as “fuel of the future” or “green fuel” and has been in news, with transport companies eager to run trains, cars and aeroplanes using biodiesel to cut down both on cost and pollution
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