Abstract
A field and glasshouse experiment was conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on growth and leaf chlorophyll content in soybean. The treatments consisted of B. japonicum inoculation (with & without), phosphorus supplementation at the levels of 0, 20, 40 and 80 kgP·ha-1. Both treatments were replicated four times in a split plot design. The following parameters were measured: plant height (cm), number leaves per plant, number of days to 50% flowering, number of days to 50% pod formation, stem girth and leaf area (LA). Results showed that inoculation with B. japonicum significantly increased plant height, number of leaves, leaf chlorophyll content, stem girth, leaf area (LA) and leaf area index (LAI). However, Phosphorus supplementation had significant effects in some parameters measured. The use of effective strain of B. japonicum and phosphorus supplementation was an efficient way of enhancing the growth of soybean.
Highlights
Nitrogen is a crucial element in the production of both leguminous and non-leguminous crops and has constructive impacts on growth of legumes [1,2]
The plant height for field experiment increased with rhizobial inoculation for the entire interval of the soybean growth as follows: At 2 weeks after planting (WAP), 4, 6 and 8WAP, the plant height increased by 16%, 35%, 51% and 29% re
Inoculated treatment showed significant increase in leaf chlorophyll content by 29% for measurements taken 4WAP for the glasshouse relative to the control
Summary
Nitrogen is a crucial element in the production of both leguminous and non-leguminous crops and has constructive impacts on growth of legumes [1,2]. It is a major constituent of chlorophyll, the most essential pigment needed for photosynthesis and amino acids. N is a building block of proteins and is highly needed for all enzymatic reactions in a plant [5] It is a major part of the chlorophyll molecules and plays a necessary role in photosynthesis and is a major component of several vitamins [6]. Nitrogen supply has significant effect on leaf growth because it increases the leaf area of plants and it influences photo-
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