Abstract
Ammonium uptake kinetics and interactions between nitrate and ammonium uptake were examined inChattonella antiqua. After the addition of ammonium to the culture ofC. antiqua, the ammonium concentration decreased linearly with time. The ammonium uptake rate as a function of ammonium concentration followed the Michaelis-Menten equation; the maximal uptake rate was 2.0 pmol cell−1hr−1 and the half saturation constant, 2.2ΜM. Although the ammonium uptake was not affected by nitrate, uptake of nitrate was rapidly (≪15min) suppressed by ammonium and a 50% reduction in nitrate uptake was observed at an ammonium concentration ofca. 2ΜM.
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