Abstract
Ammonium chloride inhibits Nosema algerae spore germination under alkaline conditions and is weakly stimulatory in the acidic pH range. By testing the inhibitory effect of solutions with equivalent concentrations of NH 3, adjusted either by pH or NH 4Cl concentration, NH 3 was shown to be responsible for inhibition of germination. Percentage germination is reduced by ammonia without slowing the germination rate. A comparison of sensitive periods for NaCl stimulation and NH 3 inhibition suggests that NH 3 exerts its inhibitory effects only during and shortly after the stimulatory phase of germination. Increased intrasporal pH caused by the protonation of NH 3 to NH 4 + after entering the spore could explain inhibition of germination by NH 3.
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