Ammonia and sodium sulfite synergistically pretreat reed to enhance enzymatic saccharification

Abstract

Pretreatment is important to overcome the structural recalcitrance of reed (a viable energy grass) to produce fermentable sugar. Herein, the study reported the pretreatment of reed using different alkali chemicals (sodium hydroxide/anthraquinone, sodium hydroxide/sodium sulfite, sodium hydroxide/sodium sulfide, ammonia/hydrogen peroxide, triethanolamine, and ammonia/sodium sulfite). The comparative study showed that the pretreatment using ammonia and sodium sulfite (NS) performed the best among them. The NS pretreatment of reed was further optimized using the Response Surface Methodology (RSM). The results showed that about 90.36% lignin was removed when reed was pretreated with 10 wt% of ammonia and 10% of sodium sulfite at 172 °C for 20 min. The excellent lignin removal performance was attributable to the synergistic effects between ammonia and sodium sulfite. The NS pretreated reed achieved 85.6% of enzymatic hydrolysis efficiency and 64.83% of total sugar yield.