Abstract
Metabolic incorporation of adenine-2-14C by Saccharomyces cerevisiae was examined for alterations produced by 3-amino-1,2,4-triazole (amitrole) and histidine. The radioactivity recovered from cells after a 4-hour incubation was found predominantly in ethanol-insoluble components (nucleic acids and proteins) of untreated cultures. Amitrole inhibited incorporation of 14C into insoluble components. Carbon-labeled imidazoleglycerol phosphate, a precursor of histidine, and labeled imidazoleglycerol accumulated in the ethanol-soluble fraction of amitrole treated cells. Imidazoleglycerol accumulated in the external medium. Histidine prevented 14C incorporation into the hot trichloroacetic acid-insoluble residue (protein), prevented amitrole-induced imidazoleglycerol and imidazoleglycerol phosphate accumulation, and circumvented amitrole inhibition of 14C incorporation into the hot trichloroacetic acid-soluble (nucleic acids) fraction.
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