AMITOSIS IN CELLS GROWING IN VITRO

Abstract

The observations above described and the interpretations thereof may be briefly summarized as follows:Amitosis was found to involve only the nucleus and was not a method of cell proliferation. It occurred in normal cells and was characterized by a separation of the nucleus into one or more parts which possessed no reproductive independence.The process of nuclear amitosis consisted in a unilateral or bilateral constriction, manifested by a narrowing of the nucleus in the region of its equator, and a streaming of its contents toward the poles, with final separation of the two nuclear portions. This phenomenon seemed to be associated with the action of the mitochondria and centrosphere upon an elongated nucleus. There was no amphiaster or spireme formation and no centrosome fission. Division of the nucleolus was not an essential. Repetition of this process leads to the formation of a giant cell.Not all nuclei which show elongation and constriction divide by direct fission, but many return to their usual rounded or oval form. When, however, the constriction has passed a critical point the division goes on to completion, and this final stage is rapid.Cells containing nuclei in process of, or the result of, amitosis divide by mitosis. Mitosis in binucleate cells, which are the product of nuclear amitosis, is characterized by the simultaneous appearance in the nuclear parts of a spireme, from which a single equatorial plate of chromosomes is formed. Furthermore, binucleate cells divide as frequently by mitosis as do mononucleate cells, and this was the only form of division found to occur in them.Since the parts of an amitotically divided nucleus do not become separated as reproductive units but divide only by mitosis, in which the chromatin in the parts is recombined, there is nothing in nuclear amitosis opposed to the chromosome hypothesis.The type of amitosis in which the nucleus is split by the growth through it from one side to the other of a membrane was not found. Nuclear figures simulating this proved to be caused by the close apposition of separate nuclear sacs, or by nucleoli, mitochondria or folds of the nuclear membrane.The dyes, janus green and gentian violet, were toxic and their presence in the cell was incompatible with its continued life. They were, however, of service in quickly studying structural details which were not discernible in the living state.Nuclear fragmentation, which differs in many ways from nuclear amitosis, is a pathological condition, and occurs in degenerating cultures.It is believed that the facts brought to light through the tissue culture method may be applied to the interpretation of the phenomena of normally developing cells.