Abstract
This work evaluated the adsorption and protection capacities of amino-functionalized mesoporous silica nanoparticles (Am-MSNs) for the recombinant DNA (rDNA), pcDNA3.1(+)-PKB-HA. The in vitro biocompatibility of Am-MSNs was also tested on HeLa and A549 cells, respectively. The results reveal that the MSNs modified with 3-aminopropyltriethoxysilane exhibit good adsorption capacity for pcDNA3.1(+)-PKB-HA at physiological conditions and the adsorption amount is higher than that of other silica nanoparticles. Moreover, the material can also protect pcDNA3.1(+)-PKB-HA from enzymatic degradation completely and presents high in vitro biocompatibility. This demonstrates that Am-MSNs may be potential nonviral vector candidates for pcDNA3.1(+)-PKB-HA.
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