Amino acid substitutions in CPC-LIKE MYB reveal residues important for protein stability in Arabidopsis roots.

Koh Yamada,Yukichi Fujikawa,Rumi Tominaga-Wada,Takuji Wada,Michiko Sasabe,Keith R Davis

doi:10.1371/journal.pone.0205522

Koh Yamada, Yukichi Fujikawa + Show 4 more

Open Access

https://doi.org/10.1371/journal.pone.0205522

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Journal: PloS one	Publication Date: Oct 11, 2018
Citations: 6	License type: CC BY 4.0

Affiliation: Hiroshima University, Hirosaki University

Abstract

TRYPTICHON (TRY) and ENHANCER OF TRY AND CPC2 (ETC2) encode R3-type MYB transcription factors that are involved in epidermal cell differentiation in Arabidopsis thaliana. TRY and ETC2 belong to the CPC-like MYB gene family, which includes seven homolog genes. Previously, we showed that among the CPC family members, TRY and ETC2 are characterized by rapid proteolysis compared with that of other members, and we demonstrated that this proteolysis is mediated by the proteasome-dependent pathway. In this study, we compared the functions of the wild-type TRY and ETC2 proteins and their amino acid-substituted versions. Our results showed that the substitution of amino acids in the C-terminal of TRY and ETC2 conferred them the ability to induce root hair formation. Furthermore, we confirmed that these mutations enhanced the stability of the TRY and ETC2 proteins. These results revealed that the amino acids, which are important for the functions of TRY and ETC2, mediate morphological pattern formation and can be useful in understanding the pathway determining the fate of root hair cells.

Highlights

The formation of root hair and non-hair cell is a well-studied model system of pattern formation in Arabidopsis thaliana [1, 2]
Previous studies have shown that the overexpression of five members of CAPRICE gene (CPC) family, including CPC, TRY, ENHANCER OF TRY AND CPC1 (ETC1), ENHANCER OF TRY AND CPC2 (ETC2), and CPC LIKE MYB3 (CPL3), generates essentially the same epidermal phenotype [10,11,12,13,14]
These five single-repeat R3 CPC-like MYB proteins are thought to determine the fate of root hair cells and inhibit the formation of trichome

Summary

Introduction

The formation of root hair and non-hair cell is a well-studied model system of pattern formation in Arabidopsis thaliana [1, 2]. CPC has six additional homologs in the Arabidopsis genome, including TRY, ENHANCER OF TRY AND CPC1 (ETC1), ETC2, ETC3/CPC LIKE MYB3 (CPL3), TRICHOMELESS1 (TCL1), and TCL2 [11,12,13,14,15,16,17,18]. These seven CPC family genes are believed to act as inducers of root hair differentiation and repressors of trichome formation [9, 18]. The CPC family genes have common characteristics in regulating the root hair and trichome development as described above, their specific functions differ [19]

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