Abstract
1. 1. The acid-soluble purine and pyrimidine compounds of a leucine auxotroph of E. coli K 12 have been fractionated of Dowex-2 formate, followed by paper chromatography and electrophoresis. 2. 2. The acid-soluble fraction contains large amounts of free hypoxanthine and uracil, as well as nucleotide co-enzymes and some nucleoside di- and tri-phosphates, but has only very small amounts of nucleoside monophosphates. Derivatives of adenine and uracil predominate. 3. 3. Leucine starvation increases the concentrations of most but not all of the bases and nucleotides. No new nucleotide compounds have been found in starved cells.
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