Abstract
Summary: To investigate the amino acid sequence determinants for the incorporation of Vpx protein into virion particles, several mutations were introduced into the vpx gene of SIVmac239 proviral DNA, and the effects of mutations on the expression and assembly of the Vpx protein were studied. The results show that deletions of amino acids from 78 to 80 or from 82 to 87 abolished the incorporation of the expressed Vpx protein into virion particles. Other Vpx mutants, including a full-length Vpx-Vpr fusion protein and a mutant with a deletion of the C-terminal polyproline tract, were packaged efficiently. This study suggests that amino acids from 78 to 80 and 82 to 87 are important for the assembly of the Vpx protein into virus particles.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Acquired Immune Deficiency Syndromes & Human Retrovirology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
