Amino Acid Profiling with Chemometric Analysis as a Feasible Tool for the Discrimination of Marine-Derived Peptide Powders.

Qin Wang,Yanchao Wang,Zhaojie Li,Lei Ma,Changhu Xue,Yaoguang Chang,Xiaoming Jiang,Yuming Wang

doi:10.3390/foods10061294

Abstract

Marine-derived peptide powders have suffered from adulteration via the substitution of lower-price peptides or the addition of adulterants in the market. This study aims to establish an effective approach for the discrimination and detection of adulterants for four representative categories of marine-derived peptide powders, namely, oyster peptides, sea cucumber peptides, Antarctic krill peptides, and fish skin peptides, based on amino acid profiling alongside chemometric analysis. The principal component analysis and orthogonal partial least squares discriminant analysis results indicate that four categories of marine-derived peptides could be distinctly classified into four clusters and aggregated with the respective raw materials. Taurine, glycine, lysine, and protein contents were the major discriminants. A reliable classification model was constructed and validated by the prediction dataset, mixture sample dataset, and unclassified sample dataset with accuracy values of 100%, 100%, and 100%, respectively.

Highlights

Marine-derived peptide powders are generally manufactured from marine organisms through protease enzymatic hydrolysis [1,2]
From the perspective of amino acid composition, the four categories of raw materials contained abundant amounts of GLU, which represented the amino acid with the highest content both in the oyster and Antarctic krill groups
This study has confirmed the feasibility of discrimination and adulteration detection of four representative categories of marine-derived peptides, including sea cucumber peptides, oyster peptides, Antarctic krill peptides, and fish skin peptides, by employing amino acid profiles combined with chemometric analysis

Summary

Introduction

Marine-derived peptide powders are generally manufactured from marine organisms through protease enzymatic hydrolysis [1,2]. Such peptide powders, mainly containing polypeptides, oligopeptides, and amino acids, are derived from protein degradation and belong to a single complex protein hydrolysate product [2,3]. Marine-derived peptides are attracting more and more attention and have been widely applied in the food, pharmaceutical, and cosmetic industries [1,7]. Echinodermata, Mollusca, Arthropoda, and Chordata, representing typical species of edible marine animals, have been widely used to produce marine-derived peptide powders

Objectives

Methods

Results

Conclusion