Amino acid profiling of Sorghum bicolor vis-à-vis Chilo partellus (Swinhoe) for biochemical interactions and plant resistance

Abstract

Amount of certain amino acids required by herbivores, and their availability in host plants are of crucial importance for insect growth, development, and life processes. Therefore, we carried out profiling and quantification of 17 amino acids in diverse sorghum genotypes, and on the Chilo partellus larvae reared on them, to understand the association and contribution of different amino acids in plant resistance to insects. Sorghum germplasm lines IS 2205 and IS 2123 had severe detrimental effects on the development and survival of C. partellus followed by varieties ICSV 700 and ICSV 708 in comparison to susceptible check, Swarna. Profiling of sorghum seedlings, seeds, and the C. partellus larvae fed on these genotypes for 17 amino acids revealed that Arginine, Glycine, Isoleucine, Leucine, Proline, and Valine in sorghum seedlings and the C. partellus larvae had significant and positive association, suggesting their role in the development and survival, while negative association of Cystine indicated its contribution in plant defense. Furthermore, C. partellus acquired less of cyclic and aliphatic amino acids per unit amount from the test resistant genotypes, while more from the susceptible check, than their presence in the seedlings. Present studies suggest that Alanine, Cystine, Glycine, and Proline contents in C. partellus larvae; Cystine and Proline contents in sorghum seedlings; and Methionine content in sorghum seeds, have significant and negative association, and contribute to explain >93% and >96%, respectively, of the variability in antibiosis mechanism and overall resistance to C. partellus. These studies have implications for antibiosis and nutritional mechanism of host plant–insect interactions in sorghum against C. partellus.