Amino Acid Profiles and Biopotentiality of Hydrolysates Obtained from Comb Penshell (Atrina pectinata) Viscera Using Subcritical Water Hydrolysis.

Jin-Seok Park,Byung-Soo Chun,Hee-Jeong Lee,Sung-Yeol Kim,Seung-Chan Lee,Yu-Rin Jeong,Truc Cong Ho,Vikash Chandra Roy

doi:10.3390/md19030137

Abstract

The recovery of amino acids and other important bioactive compounds from the comb penshell (Atrina pectinata) using subcritical water hydrolysis was performed. A wide range of extraction temperatures from 140 to 290 °C was used to evaluate the release of proteins and amino acids. The amount of crude protein was the highest (36.14 ± 1.39 mg bovine serum albumin/g) at 200 °C, whereas a further increase in temperature showed the degradation of the crude protein content. The highest amount of amino acids (74.80 mg/g) was at 230 °C, indicating that the temperature range of 170–230 °C is suitable for the extraction of protein-rich compounds using subcritical water hydrolysis. Molecular weights of the peptides obtained from comb penshell viscera decreased with the increasing temperature. SDS-PAGE revealed that the molecular weight of peptides present in the hydrolysates above the 200 °C extraction temperature was ≤ 1000 Da. Radical scavenging activities were analyzed to evaluate the antioxidant activities of the hydrolysates. A. pectinata hydrolysates also showed a particularly good antihypertensive activity, proving that this raw material can be an effective source of amino acids and marine bioactive peptides.

Highlights

IntroductionThe comb penshell, Atrina pectinata, is a marine bivalve belonging to the phylum Mollusca, and is found mostly in subtidal areas of the Indian and Western Pacific Oceans [1,2,3]
The comb penshell, Atrina pectinata, is a marine bivalve belonging to the phylum Mollusca, and is found mostly in subtidal areas of the Indian and Western Pacific Oceans [1,2,3].South Korea has reported five species of the comb penshell; among these, thousands of tons of A. pectinata are harvested every year [4]
The raw material showed 60.70% ± 0.11% crude protein, which indicates that it was suitable for the extraction of peptides and amino acids

Summary

Introduction

The comb penshell, Atrina pectinata, is a marine bivalve belonging to the phylum Mollusca, and is found mostly in subtidal areas of the Indian and Western Pacific Oceans [1,2,3]. South Korea has reported five species of the comb penshell; among these, thousands of tons of A. pectinata are harvested every year [4]. A large amount of remaining parts, such as the viscera (approximately 25% of the total body weight), are generated as waste in bivalve-processing factories [5]. From the consumption pattern of the comb penshell, of which only its muscle is largely eaten, the remaining viscera and shell can be a valuable source of obtaining different biopolymers.

Methods

Results

Conclusion