Amino acid incorporation into proteins of human malignant cervical tissue.

Abstract

AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Protein synthesis in subcellular fractions from normal and malignant cervix uteri has been investigated in regard to amino acid incorporation, which takes place more actively in microsomal and mitochondrial systems in the malignant tissue than in normal tissue. Incorporating activities of mitochondia and microsomes can be considerably stepped up, using poly U or poly A for the corresponding labelled amino acids and greatly suppressed by mitomycin C, emetine, and “Jawaharene,” a fungal antibiotic. References 1 Ashwell, G.: Determination of pentoses. In Methods in Enzymology, vol. 3, S. P. Collowick and N. O. Kaplan, Eds. New York, Academic Press. 1955; pp. 87– 90. 2 Banerjee, A. K., Banerjee, Sipra, and Roy, S. C.: Studies in human malignant tissue. I. Protein synthesis in malignant and normal cervix uteri. Indian J. Biochem. 2: 109– 112, 1965. 3 Banerjee, Sipra, and Roy, S. C.: Studies in human malignant tissues. II—Incorporation of L–leucine–14C into mitochondrial protein of human normal and malignant cervix uteri. Indian J. Biochem. 3: 114– 118, 1965. 3 Burton, K.: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62: 315– 323, 1956. 4 Colombo, B., Felicetti, L., and Baglioni, C.: Inhibition of protein synthesis in reticulocytes by antibiotics. Biochim. Biophys. Acta 119: 109– 119, 1966. 5 Das, H. K., Chatterjee, S. K., and Roy, S. C.: Protein synthesis in plant mitochondria. J. Biol. Chem. 239: 1126– 1133, 1964. 6 Ennis, H. L., and Lubin, M.: Cycloheximide; Aspects of inhibitors of protein synthesis in mammalian cells. Science 146: 1474– 1476, 1964. 7 Fiske, C. H., and SubbaRow, Y.: The colorimetric determination of phosphorus. J. Biol. Chem. 66: 375– 400, 1925. 8 Griffin, A. C.: In vitro studies on protein synthesis by malignant cells. In Advances in Cancer Research, vol. 10, Alexander Haddow and Sidney Weinhouse, Eds. New York, Academic Press. 1967; pp. 83– 116. 9 Grollman, A. P.: Inhibitors of protein biosynthesis. J. Biol. Chem. 243: 4089– 4094, 1968. 10 Grollman, A. I.: Emetine in the treatment of intraabdominal and ritsoperitonial nonspecific Granulomas. Surg. Gynecol. Obstet. 120: 792– 796, 1965. 11 Gronall, A. G., Barderwill, C. S., and David, M. M.: Determination serum protein by means of the biuret reaction. J. Biol. Chem. 177: 751– 766, 1949. 12 Hata, T., Hossenlopp, C., and Takita, H.: Studies on mitomycin C, specially method of administration. Cancer Chemother. Rep. 13: 67– 77, 1961. 13 Hoagland, M. B., Keller, E. B., and Zamecnik, P. C.: Enzymatic carboxyl activation of amino acids. J. Biol. Chem. 218: 345– 358, 1950. 14 Johnson, F.: Relationship of structure to fungi toxicity of cycloheximide and related glutarimide derivatives. Biochem. Pharmacol. 15: 1213– 1223, 1966. 15 Kroon, A. M.: Amino acid incorporation to the protein of mitochondria and mitochondrial fragments from beef heart. Biochim. Biophys. Acta 69: 184– 185, 1963. 16 Lerman, M., and Benyumovich, M. S.: Effect of mitomycin C on protein synthesis in human neoplastic cell lines. Nature (London) 206: 1231– 1232, 1965. 17 McLean, J. R., Cohn, G. L., Brandit, I. K., and Simpson, M. V.: Incorporation of labelled amino acids into the protein of muscle and liver mitochondria. J. Biol. Chem. 233: 657– 663, 1958. 18 Roy, D. K.: Protective action of Jawaharene in vaccina infection of Rabbits. Naturwissenschaften 50: 501– 503, 1963. 19 Schneider, W. C.: Phosphrous compounds in animal tissues. J. Biol. Chem. 161: 293– 303, 1945. 20 Schneider, W. C., and Hogeboom, G. H.: Intracellular distribution of enzymes. J. Biol. Chem. 183: 123– 128, 1950. 21 Sisler, H. D., and Siegel, M. R.: Cycloheximide and gluterimide antibiotics. In Antibiotics, vol. 1, D. Gottlieb and P. D. Shaw, Eds. Berlin and New York, Springer–Verlag, 1967; pp. 283– 303. 22 Stachiewicz, E., and Quastel, J. H.: Effects of dihydrostreptomycin on amino acid incorporation into the proteins of M. tuberculosis (B. C. G.). Canad. J. Biochem. Physiol. 37: 687– 697, 1959. 23 Truman, D. E. S., and Korner, A.: Incorporation of amino acids into the protein of isolated mitochondria. Biochem. J. 83: 588– 596, 1962. 24 Zamecnik, P. C., Loftfield, R. B., Stephenson, M. L., and Steele, J. M.: Studies on the carbohydrate and protein metabolism of the rat hepatoma. Cancer Res. 11: 592– 602, 1951. Citing Literature Volume29, Issue6June 1972Pages 1575-1578 ReferencesRelatedInformation